MAPs Elution Buffer

50 mm PIPES (pH 6.9)

2 mm EGTA

1 mm MgSO₄

1 mm DTT (or DTE)

0.1 mm GTP

0.8 M NaCl or KCl

The salt for elution should contain the same cation as is present in the base (KOH or NaOH) used to titrate the phosphocellulose when preparing the column.

• © 2015 Cold Spring Harbor Laboratory Press