Analysis of Spine Motility of Newborn Granule Cells in Acute Brain Slices
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
In this protocol, acute brain slices are prepared from mice in which newborn granule cells have been labeled using retroviral vector technology. Using a live-cell imaging stage and confocal microscopy coupled to imaging software, dendritic spines are analyzed.
- © 2015 Cold Spring Harbor Laboratory Press
