Yeast Presporulation Medium
| Reagent | Final concentration |
|---|---|
| Bacto yeast extract | 0.5% (w/v) |
| Bacto peptone | 1% (w/v) |
| Potassium acetate | 1% (w/v) |
| Yeast nitrogen base without amino acids and ammonium sulfate | 0.17% (w/v) |
| Ammonium sulfate | 0.5% (w/v) |
| Potassium hydrogen phthalate | 0.05 m |
| Adjust the pH to 5.5 with 10 n KOH, add 20 μL of antifoam agent (polypropyleneglycol 2000) per liter of solution, and autoclave. | |
- © 2015 Cold Spring Harbor Laboratory Press
