Loading Dye (6×)
| Reagent | Final concentration |
|---|---|
| Bromophenol blue | 0.25% (w/v) |
| Xylene cyanol | 0.25% (w/v) |
| Ficoll 400 | 15% (w/v) |
| Dissolve the reagents in water. Mix thoroughly, filter sterilize, and store at 4°C. | |
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| Reagent | Final concentration |
|---|---|
| Bromophenol blue | 0.25% (w/v) |
| Xylene cyanol | 0.25% (w/v) |
| Ficoll 400 | 15% (w/v) |
| Dissolve the reagents in water. Mix thoroughly, filter sterilize, and store at 4°C. | |