Recipe

Lysis Buffer for Yeast Spheroplasts

Reagent Final concentration
Tris–HCl (pH 8.0)    100 mm
EDTA (pH 8.0)    50 mm
SDS 0.5% (w/v)
Make fresh on day of DNA isolation. Store at room temperature.
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  1. doi:10.1101/pdb.rec088492 Cold Spring Harb Protoc 2015: pdb.rec088492- © 2015 Cold Spring Harbor Laboratory Press

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