Zymolyase Buffer
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Sorbitol (2 m) | 50 mL | 1 m |
| Potassium phosphate (pH 7.5) | 4.2 mL of 1 m K2HPO4 0.8 mL of 1 m KH2PO4 |
50 mm |
| EDTA (0.5 m, pH 8.0) | 1 mL | 5 mm |
| Bring to volume with deionized water and filter sterilize. Prepare aliquots and store at −20°C. | ||
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