Recipe

Dissection Buffer

KCl  2.5 mm
NaH2PO4 • H2O  1.3 mm
NaHCO3   25 mm
Dextrose   20 mm
Ascorbate  1.3 mm
Pyruvate  0.6 mm
Choline 110 mm
Kynurenic acid (stock solution of kynurenic acid is made with 1 m NaOH)  5.5 mm
CaCl2  0.5 mm
MgCl2  7.0 mm
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  1. doi:10.1101/pdb.rec089672 Cold Spring Harb Protoc 2015: pdb.rec089672- © 2015 Cold Spring Harbor Laboratory Press
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