Dissociated Prostate Regeneration under the Renal Capsule
- 1Howard Hughes Medical Institute, University of California, Los Angeles, California 90095;
- 2Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095;
- 3Department of Urology, University of California, Los Angeles, California 90095;
- 4Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095;
- 5Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, California 90095;
- 6Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, California 90095
Abstract
Tissue recombination models are useful for studying cancer initiation, progression, and metastasis. They also provide an in vivo environment in which to investigate the functional role of stem cells in tissue repair. In this protocol, we describe in detail the dissociated prostate regeneration assay. Dissociated adult murine prostate cells are combined with embryonic urogenital sinus mesenchymal cells and implanted under the renal capsule. Morphological tissue structures with appropriate epithelial–stroma interactions are reconstituted in the grafts.
Footnotes
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↵7 Correspondence: andrew.goldstein{at}ucla.edu
- © 2015 Cold Spring Harbor Laboratory Press
