Cathepsin Reaction Buffer
| Reagent | Final concentration |
|---|---|
| Sodium acetate | 50 mm |
| EDTA | 4 mm |
| Pefabloc | 0.5 mm |
| Dithiothreitol (DTT) | 8 mm |
| Z-FR-AFC substrate (MP Bio) | 50 µm |
| Prepare a stock solution that contains the first two ingredients, adjust pH to 6.0, and store it at 4°C. Immediately before use, add the last three ingredients. | |
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