Recipe

Spheroplast Solution

Reagent Final concentration
Potassium phosphate (pH 7.5)    50 mm
EDTA    10 mm
Sorbitol     1.2 m
Zymolyase 100T (e.g., MP Biomedicals) 0.25 mg/mL
2-Mercaptoethanol      1%
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  1. doi:10.1101/pdb.rec088286 Cold Spring Harb Protoc 2015: pdb.rec088286- © 2015 Cold Spring Harbor Laboratory Press
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