Recipe

Modified Artificial Cerebrospinal Fluid (ACSF)

Reagent Final concentration
Sucrose  250 mm
KCl   3 mm
CaCl2   2 mm
MgSO4   2 mm
NaH2PO4   1.25 mm
NaHCO3   27 mm
Dextrose   10 mm
Saturate the solution with 95% O2 and 5% CO2.
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  1. doi:10.1101/pdb.rec090092 Cold Spring Harb Protoc 2015: pdb.rec090092- © 2015 Cold Spring Harbor Laboratory Press
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