Recipe

Column Buffer for Sephacryl S-300 (Salt-Free)

5% (w/v) sucrose

0.1% (w/v) CHAPS

20 mm HEPES

5 mm dithiothreitol (DTT)

Adjust to pH 7.5. Filter at 0.45 µm.

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  1. doi:10.1101/pdb.rec087742 Cold Spring Harb Protoc 2015: pdb.rec087742- © 2015 Cold Spring Harbor Laboratory Press
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