Recipe

DISC Lysis Buffer

30 mm Tris/HCl (pH 7.5)

150 mm NaCl

10% glycerol

1% Triton X-100

Complete protease inhibitors (Roche)

Store buffer at 4°C.

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  1. doi:10.1101/pdb.rec087767 Cold Spring Harb Protoc 2015: pdb.rec087767- © 2015 Cold Spring Harbor Laboratory Press
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