Recipe

PIPES Buffer

50 mm PIPES/KOH

2 mm EDTA

0.1% (w/v) CHAPS

5 mm dithiothreitol (DTT)

10 µg/mL aprotinin

20 µg/mL leupeptin

10 µg/mL pepstatin A

2 mm phenylmethylsulfonyl fluoride (PMSF)

Adjust to pH 6.5.

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  1. doi:10.1101/pdb.rec087775 Cold Spring Harb Protoc 2015: pdb.rec087775- © 2015 Cold Spring Harbor Laboratory Press
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