Recipe

SDS-PAGE Sample Loading Buffer (4×)

250 mm Tris–HCl (pH 6.8)

8% (w/v) sodium dodecyl sulfate (SDS)

0.2% (w/v) bromophenol blue

40% (v/v) glycerol

20% (v/v) β-mercaptoethanol

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  1. doi:10.1101/pdb.rec087791 Cold Spring Harb Protoc 2015: pdb.rec087791- © 2015 Cold Spring Harbor Laboratory Press
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