Total Cellular Ca2+ Measurements in Yeast Using Flame Photometry
- 1Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Milan, Italy;
- 2Laboratório de Bioquímica e Biotecnologia de Leveduras, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, 35.400-000 Ouro Preto, Minas Gerais, Brazil
Abstract
A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.
Footnotes
-
↵3 Correspondence: renata.tisi{at}unimib.it
- © 2015 Cold Spring Harbor Laboratory Press
