Recipe

Valap Sealant

Add equal weight of petroleum jelly (e.g., Vaseline), lanolin, and paraffin together in a 1-L beaker. (The preparation of a few hundred milliliters of this mixture should be a lifetime supply.) Heat the mixture at a low setting on a hot plate. Stir occasionally until thoroughly blended. Take extreme care not to overheat this mixture. Warm it just enough to make the components liquefy. The final product should be golden yellow, the color of corn or canola oil. If it is dark brown, it was heated too much. In that case, start again. Aliquot the final mixture into several small screw-capped jars (∼50-mL capacity).

Store at room temperature. Keep a small amount in a 25-mL beaker, and warm at a low setting on a hot plate before use.

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  1. doi:10.1101/pdb.rec082917 Cold Spring Harb Protoc 2015: pdb.rec082917- © 2015 Cold Spring Harbor Laboratory Press

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