Recipe

Tracking Dye

Reagent Quantity (for 1 mL) Final concentration
MOPS buffer (10×) 100 µL 1×
Ficoll 400 150 mg   15%
Bromophenol blue (1%) H2O    200 µL–1 mL  0.2%
Store for up to 1 yr at −20°C.

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  1. doi:10.1101/pdb.rec083568 Cold Spring Harb Protoc 2015: pdb.rec083568- © 2015 Cold Spring Harbor Laboratory Press
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