Recipe

Bead-Binding Buffer

Phosphate-buffered saline (PBS)

Tween 20 (0.2%)

Dissolve one pouch of powdered PBS (Sigma-Aldrich P5368) in 1 L of dH2O, and sterilize by autoclaving. Add Tween 20 to a final concentration of 0.2%, and filter-sterilize. Store for up to 1 yr at 4°C.

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  1. doi:10.1101/pdb.rec085480 Cold Spring Harb Protoc 2015: pdb.rec085480- © 2015 Cold Spring Harbor Laboratory Press

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