ChIP-Seq Dilution Buffer

0.01% SDS

1.1% Triton X-100

1.2 mm EDTA

16.7 mm Tris (pH 8.1)

167 mm NaCl

Filter-sterilize. Store for up to 1 yr at 4°C. Add one protease inhibitor tablet or 2 mL of 25× inhibitor stock solution as directed by the manufacturer (cOmplete EDTA-Free; Roche) per 50 mL of buffer immediately before use.

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