ChIP-Seq Wash Buffer

100 mm Tris (pH 8.0)

500 mm LiCl (or NaCl, for a lower-stringency wash)

1% NP-40

1% deoxycholic acid

Filter-sterilize. Store for up to 1 yr at 4°C. Add one protease inhibitor tablet or 2 mL of 25× inhibitor stock solution as directed by the manufacturer (cOmplete EDTA-Free; Roche) per 50 mL of buffer immediately before use.

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