ChIP-Seq Nuclear Lysis Buffer

10 mm Tris (pH 8.0)

1 mm EDTA

0.5 mm EGTA

0.2% SDS concentration can be increased to (0.5% SDS as required)

Filter-sterilize. Store for up to 1 yr at 4°C. Add one protease inhibitor tablet or 2 mL of 25× inhibitor stock solution as directed by the manufacturer (cOmplete EDTA-Free; Roche) per 50 mL of buffer immediately before use.

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