High-Salt Sucrose Cushion Buffer for Prokaryotic Ribosomes

20 mm Tris-HCl (pH 7.5)

50 mm MgOAc

100 mm NH₄Cl

1 mm DTT

0.5 mm EDTA

1.1 m sucrose (certified RNase-free)

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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