Recipe

Plant Extraction Buffer

50 mm Tris-HCl (pH 9.0)

30 mm MgCl2

400 mm KCl

17% (w/w) sucrose (certified RNase-free)

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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  1. doi:10.1101/pdb.rec081596 Cold Spring Harb Protoc 2015: pdb.rec081596- © 2015 Cold Spring Harbor Laboratory Press
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