Plant Extraction Buffer
50 mm Tris-HCl (pH 9.0)
30 mm MgCl2
400 mm KCl
17% (w/w) sucrose (certified RNase-free)
Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.
- © 2015 Cold Spring Harbor Laboratory Press
