Mammalian Sucrose Cushion Buffer
50 mm Tris-HCl (pH 7.5)
5 mm MgCl2
25 mm KCl
2 m sucrose (certified RNase-free)
Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.
- © 2015 Cold Spring Harbor Laboratory Press
