Recipe

Tight Couples Buffer

10 mm Tris-HCl (pH 7.5)

6 mm MgOAc

50 mm NH4Cl

1 mm DTT

0.5 mm EDTA

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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  1. doi:10.1101/pdb.rec081646 Cold Spring Harb Protoc 2015: pdb.rec081646- © 2015 Cold Spring Harbor Laboratory Press
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