Recipe

Probe Hybridization Buffer for HCR

50% formamide, deionized (Ambion AM9342)

5× SSC (Invitrogen/Life Technologies 15557-044)

9 mm citric acid (pH 6.0) (Sigma-Aldrich C2404)

0.1% Tween 20 (Bio-Rad 170-6531)

50 µg/mL heparin (Sigma-Aldrich H3393)

1× Denhardt's solution (Invitrogen/Life Technologies 750018)

10% dextran sulfate (Sigma-Aldrich D6001)

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  1. doi:10.1101/pdb.rec083899 Cold Spring Harb Protoc 2015: pdb.rec083899- © 2015 Cold Spring Harbor Laboratory Press
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