SLAHD Agar Plates
2% glucose
6.7 g/L yeast nitrogen base without amino acids and ammonium sulfate
20 mg/L uracil
20 mg/L histidine or 500 mg/L proline
20 g/L agar
Histidine or proline can be used interchangeably, depending on the genotype of the yeast strain used. Include any additional amino acids that may be required for the growth of an auxotrophic strain. Prepare in H2O and sterilize by autoclaving. Fill sterile Petri dishes with ∼25 mL of autoclaved medium.
- © 2015 Cold Spring Harbor Laboratory Press
