Recipe

Sodium Phosphate Buffer for Church and Gilbert Hybridization

Reagent Quantity (for 1 L)
Na2HPO4 · 7H2O  134 g
H3PO4 (85%)  4 mL
H2O  to 1 L
The pH of this solution must be adjusted with phosphoric acid (Church and Gilbert 1984). Store indefinitely at room temperature.

REFERENCES

    1. Church GM,
    2. Gilbert W.
    Church GM, Gilbert W. 1984. Genomic sequencing. Proc Natl Acad Sci 81: 1991–1995.
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  1. doi:10.1101/pdb.rec086579 Cold Spring Harb Protoc 2015: pdb.rec086579- © 2015 Cold Spring Harbor Laboratory Press
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