Table 1.
Effect of electroporation of C6 glioma cells with a field strength of 2000 V/cm on cell death quantified by various markers
| % of dead cells (± s.e.m.) | |||
|---|---|---|---|
| Cell death event | Cell death marker | Electroporated | Nonelectroporated |
| Caspase activationa | FITC-VAD-FMK in situ marker | 1.33 ± 0.6 | 1.14 ± 0.24 (n = 5) |
| Condensed nucleia | DAPI staining | 0.37 ± 0.09 | 0.22 ± 0.05 (n = 5) |
| Membrane permeabilityb (primary/secondary necrosis) |
Propidium iodide uptake | 0.83 ± 0.23 | 0.58 ± 0.21 (n = 3) |
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a Cell death investigated 6 h after in situ electroporation.
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b Cell death investigated 1 h after in situ electroporation; cell death was examined in C6 glioma cultures.
