Topic Introduction

Multiphoton Excitation of Fluorescent Probes

  1. Warren R. Zipfel
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

This introduction reviews the multiphoton excitation cross sections of extrinsic and intrinsic fluorophores, genetically engineered probes, and nanoparticles. We will review the known two-photon excitation cross sections of biological indicators and will discuss several related issues such as how to theoretically estimate and experimentally gauge the two-photon cross section of an indicator. We provide practical guides for experimentally estimating the excitation cross section.

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  1. doi:10.1101/pdb.top086116 Cold Spring Harb Protoc 2015: pdb.top086116- © 2015 Cold Spring Harbor Laboratory Press
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