Dissociation of Ribosomes into Large and Small Subunits
- 1Department of Biology and Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284;
- 2Primary Pharmacology Group, Pfizer Global Research and Development, Groton, Connecticut 06340;
- 3Department of Molecular, Cell and Developmental Biology and Department of Human Genetics, University of California, Los Angeles, California 90095
Abstract
Structural and functional studies of ribosomal subunits require the dissociation of intact ribosomes into individual small and large ribosomal subunits. The dissociation of the prokaryotic 70S ribosomes into the 50S and 30S subunits is achieved by dialysis against a buffer containing a lower Mg2+ concentration. Eukaryotic 80S ribosomes are dissociated into 60S and 40S subunits by incubation in a buffer containing puromycin and higher KCl and Mg2+ concentrations.
Footnotes
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↵4 Correspondence: mcrivera{at}vcu.edu
- © 2015 Cold Spring Harbor Laboratory Press
