Solution B for Organotypic Culture
| Reagent | Volume |
|---|---|
| Eagle's minimal essential medium (EMEM; 10×, Lonza 12-684F) | 1.8 mL |
| Fetal bovine serum | 2 mL |
| l-Glutamine (200 mm; Cellgro 25-005CI) | 160 µL |
| Sodium bicarbonate (NaHCO3; Lonza 17-613E) | 380 µL |
| Bovine collagen type I (Organogenesis 200-055) | 11.4 mL |
| Matrigel (BD Biosciences 354234) | 3.8 mL |
| Fibroblasts (6 × 105 cells/mL) | 1.6 mL |
| Combine the reagents in the order listed. The final volume is sufficient for six inserts. | |
- © 2015 Cold Spring Harbor Laboratory Press
