| Aspartic acid |
130 |
| KOH |
∼130 |
| NaCl |
10 |
| MgCl2 |
0.5 |
| MgATP |
5 |
| K5Fluo-3
|
0.05 |
| K-HEPES |
10 |
| Adjust the pH of this solution to 7.2 during the titration of aspartic acid with KOH. This solution can be prepared before
the experiments and can be stored and kept at –20°C for several weeks. However, it is customary to aliquot and store the solution
without the K5Fluo-3, which is added to the pipette solution just before use. (Note that Fluo-3 will buffer contaminating Ca2+ from reagents, so if less Fluo-3 is used, additional buffer [EGTA] should be added.)
|
