Recipe

Sucrose (60%)

Add 30 g of sucrose (Sigma-Aldrich S9378) to each of two 50-mL conical tubes and bring to a total volume of 50-mL per tube with Dulbecco's phosphate-buffered saline (DPBS) (Life Technologies 14190-250). Agitate until sucrose is dissolved; this will be a lengthy process, so placing on an orbital shaker or taping to a vortex is recommended. Once dissolved, filter-sterilize using a Steriflip filter (Millipore SE1M003M00). (A single filter can be used for both tubes.) Uncontaminated solution can be stored at 4°C indefinitely.

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  1. doi:10.1101/pdb.rec080549 Cold Spring Harb Protoc 2015: pdb.rec080549- © 2015 Cold Spring Harbor Laboratory Press
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