Recipe

Prokaryotic Ribosome Dissociation Buffer

10 mm Tris-HCl (pH 7.5)

1 mm MgOAc

100 mm NH4Cl

1 mm DTT

0.5 mm EDTA

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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  1. doi:10.1101/pdb.rec081687 Cold Spring Harb Protoc 2015: pdb.rec081687- © 2015 Cold Spring Harbor Laboratory Press

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