Recipe

Mammalian Ribosome Dissociation Buffer

50 mm triethanolamine-HCl (pH 7.5)

5 mm MgCl2

500 mm KCl

1 mm puromycin

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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  1. doi:10.1101/pdb.rec081752 Cold Spring Harb Protoc 2015: pdb.rec081752- © 2015 Cold Spring Harbor Laboratory Press
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