Table 1.

Y2H bait and prey vectors and their properties (see also Fig. 1)

Gal4-Fusion Selection
Vector Promotera DBD AD Yeast Bacterial Ori Source
Baits pGBKCg t-ADH1 Carboxy-terminal – Trp1 Kanamycin 2µ Stellberger et al. 2010
pGBGT7g t-ADH1 Amino-terminal – Trp1 Gentamycin 2µ Rajagopala et al. 2014
Preys pGADCg fl-ADH1 – Carboxy-terminal Leu2 Ampicillin 2µ Stellberger et al. 2010
pGADT7g fl-ADH1 – Amino-terminal Leu2 Ampicillin 2µ Chen et al. 2010

  • at-ADH1, fl-ADH1 = truncated or full-length ADH1 promoter.

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