Digestion Buffer for Mammary Glands

25 mm HEPES

150 U/mL Collagenase type 3 (Worthington CLS-3)

20 µg/mL Liberase Blendzyme 2 (Roche 11988425001)

Prepare in DMEM/F12 containing l-glutamine (e.g., DMEM: F12 with l-glutamine, 15 mm HEPES, and 3.151 g/L glucose, LONZA 12-719F) containing 25 mm HEPES (final)

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