Recipe

K-Aspartate-Based Internal Pipette Solution

Reagent Final concentration (mm)
K-aspartate  120
NaCl   10
KCl   10
MgCl2  0.5
MgATP   5
K-HEPES   10
K5Fluo-4 0.05
Adjust the pH of this solution to 7.2 with KOH. This solution can be prepared before the experiments and can be stored at −20°C for several weeks.

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  1. doi:10.1101/pdb.rec079640 Cold Spring Harb Protoc 2015: pdb.rec079640- © 2015 Cold Spring Harbor Laboratory Press
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