Components of the stimulation paradigm. (A) The output of a function generator programmed to produce a typical 20-Hz, 5-msec pulse width, 5-V square pulse pattern is diagrammed. (B) The common variable names of these parameters are indicated in the focus on two pulses from the example pulse pattern (top). Resulting laser output (middle) should only occur during square pulses of the stimulation train at a predetermined frequency (bottom).