Protocol

Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Nick-End Labeling (TUNEL) for Detection of Apoptotic Cells in Drosophila

  1. Sharad Kumar1
  1. Centre for Cancer Biology, University of South Australia, Adelaide, South Australia 5001, Australia

    Abstract

    A characteristic feature of apoptosis is DNA fragmentation. This fragmentation can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) of DNA in dying cells. Here, we present a protocol for TUNEL detection of apoptosis in Drosophila larval tissue, but these techniques can be adapted for other tissues and developmental stages.

    Footnotes

    • ↵1 Correspondence: Donna.Denton{at}unisa.edu.au; Sharad.Kumar{at}unisa.edu.au

    | Table of Contents

    This Article

    1. doi:10.1101/pdb.prot086199 Cold Spring Harb Protoc 2015: pdb.prot086199- © 2015 Cold Spring Harbor Laboratory Press
    1. » Abstract
    2. Full Text
    3. Full Text (PDF)

    Article Category

    1. Protocol

    Personal Folder

    1. Save to Personal Folders

    Updates/Comments

    1. Submit Updates/Comments
    2. No Updates/Comments published
    3. Alert me when Updates/Comments are published

    Share

    Current Issue

    1. January 2026, 2026 (1)
    1. Current Issue