Ligation Buffer for 3C (10×)
| Reagent | Amount to add (for 1 L) | Final concentration (10×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 500 mL | 500 mm |
| MgCl2 (1 m) | 100 mL | 100 mm |
| Dithiothreitol (DTT) (1 m) | 100 mL | 100 mm |
| Prepare in deionized H2O. Store at −20°C in 15-mL aliquots. | ||
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