A + G Reaction Buffer
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| NaOAc (1 m, pH 4.5) | 5 mL | 50 mm |
| EDTA (0.5 m, pH 8.0) | 0.2 mL | 1 mm |
| H2O | 94.8 mL | |
| Adjust pH to 4.5, if necessary, before adding all of the H2O. | ||
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