Binding Buffer (BB)
| Reagent | Amount to add (for 100 mL) | Final concentration (1×) |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 5.0 mm |
| EDTA (500 mm) | 100 µL | 0.5 mm |
| NaCl (5 m) | 20 mL | 1 m |
| For 2× BB, double the volume of each reagent added. Prepare in deionized H2O. Store for up to 1 yr at room temperature. | ||
- © 2015 Cold Spring Harbor Laboratory Press
