Tween Wash Buffer (TWB)
| Reagent | Amount to add (for 100 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 5.0 mm |
| EDTA (500 mm) | 100 µL | 0.5 mm |
| NaCl (5 m) | 20 mL | 1 m |
| Tween 20 (100%) | 50.0 µL | 0.05% |
| Prepare in deionized H2O. Store for up to 1 yr at room temperature. | ||
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