Caspase Assay Lysis Buffer
| Reagent | Final concentration |
|---|---|
| HEPES (pH 7.5) | 50 mm |
| NaCl | 100 mm |
| EDTA | 1 mm |
| CHAPS | 0.1% |
| Sucrose | 10% |
| DTT | 5 mm |
| Triton X-100 | 0.5% |
| Glycerol | 4% |
| Protease inhibitor cocktail (cOmplete, Roche) | 1× |
| After adding the DTT and protease inhibitor cocktail, the lysis buffer can be stored at –20°C in 10-mL aliquots for up to 1 yr. | |
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