Recipe

SDS-PAGE Laemmli Buffer, 5×

Tris–HCl (pH 6.8) 312.5 mm
SDS   10%
Glycerol   25%
Bromophenol blue    0.5%
Add 10% β-Mercaptoethanol before use.
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  1. doi:10.1101/pdb.rec087924 Cold Spring Harb Protoc 2015: pdb.rec087924- © 2015 Cold Spring Harbor Laboratory Press
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