Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function
- Lea M. Starita1,4 and
- Stanley Fields1,2,3,4
- 1Department of Genome Sciences, University of Washington, Seattle, Washington 98195;
- 2Department of Medicine, University of Washington, Seattle, Washington 98195;
- 3Howard Hughes Medical Institute, Seattle, Washington 98195
Abstract
Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 105 or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.
Footnotes
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↵4 Correspondence: fields{at}uw.edu; lstarita{at}uw.edu
- © 2015 Cold Spring Harbor Laboratory Press
